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COMPARATIVE ANALYSIS OF CONSERVED SEQUENCES IN SWINE FLU INFECTED INDIVIDUALS

Namitha, J.N, Pavithra, N and Smitha, S.
Jayarama Reddy*, Niranjan, V**
*St. Joseph’s Post Graduate Centre, 36, Langford Road, Bangalore – 560027, India.
**Outsourse Bioinformatics, R.A.Puram, Chennai-600028, India.
*corresponding authors

ABSTRACT

Swine influenza virus (SIV) is any strain of the influenza family of viruses that is endemic in pigs. Swine influenza is known to be caused by influenza A subtypes H1N1, H1N2, H2N3, H3N1, and H3N2. Swine influenza is an infection by any one of several types of swine influenza virus. Most work has been carried out in the European and American Populations which may or may not be effective in our tropical Indian Climate. Hence, further research is needed. Also, the research done has yielded a vaccine that is not known to be effective enough. Influenza research involves investigating molecular virology, pathogenesis, host immune responses, genomics and epidemiology. The main goal of this research is to develop vaccine for Indian influenza A virus using in-silico approach involving the following steps. Firstly, More than 1 lakh sequenced influenza A genomic segments were taken for finding the consensus sequence, as these would be the potential pathogenic segments for which vaccine development have to be raised. The consensus sequence at the nucleotide level were translated to the proteomic level. The consensus were further analyzed for its antigenic properties. The Consensus antigenic peptides which had interacted with human receptors were taken and analysed for mutations. Those segments without mutation would be the peptides of interest for the vaccine development and this is further analyzed for its sub cellular localization. Based on all of the above filters, we found 16 novel proteins identified with this consensus sequence which may be of interest for finding the novel gene in influenza A. At the same time, four novel genes are predicted with the consensus antigenic peptide motif from the Indian genomic segments which can be further explored for therapeutic interest.

KEYWORDS: vaccine, swine flu, epidemiology, immunogenicity, antigen.

∙•♣♥ Love Letter ♥♣•∙

∙•♣♥ Love Letter ♥♣•∙

Dear,

I miss you darling! You were the king of this house. You stole my heart from the day I saw you. Your presence was always warm and your look would always bring a smile on my face. You were such a sweetheart that you were my constant companion. I could talk to you for hours and you still lent me your ears. You would let me tickle you, pinch you and even punch you! Your favorite food was everything, from candy to spicy biryani and even the occasional greens! Your favorite place was everywhere! You loved life!

You could say we grew up together, 14 years… that is an unbelievably long time! I’m sorry for everything that I did or didn’t do that made you unhappy. I hate myself for that!! If given a second chance, I would definitely not let a heartbeat of unhappiness in your life!

You always looked just the way you looked when I first saw you, a very handsome hunk!! You were a prince, someone who swept everyone off their feet!! By the way, your rival for Suzy’s love, still didn’t realize something is amiss. He still keeps looking into our house looking for you, I think!

I too still keep looking out in the shadows of the night, hoping to see your sparkling eyes in the moonlight! Your scent still lingers in the air and sometimes I hear a breath. Conversations run empty ending in tears. Home doesn’t feel as secure, I double lock the door. Nobody mentions your name as the pain is unbearable!! I can’t stop the tears from flowing knowing you are not here with us anymore but I console myself with the thought that you deserved a better life. I’m happy you are in a better place and don’t you ever think I will replace you in my heart! Though I never tied you with a leash, you are tightly chained in my thoughts. You were never like a dog in the house, more of a human-like family member. Baby, I hope you are fine Just thought I’d let you know you are greatly missed!

With loads of love and kisses and hugs!!